Acculturation, Bilateral Hostility, and Psychological Wellbeing of U.S.-dwelling Chinese during the COVID-19 Pandemic.

The COVID-19 pandemic has sparked hostility against Chinese immigrants and sojourners in the U.S. and other countries. Making the situation worse, strong resentment against this group has also emerged in China due to the fear of returnees spreading the disease. Integrating research on acculturation and hostile media perception, we examined how such bilateral hostility along with different acculturation components (i.e., cultural identification, COVID-19-related media use, and individualistic-collectivistic value) influenced U.S.-dwelling Chinese's psychological well-being during the COVID-19 pandemic. Based on the results from a two-wave survey assessing the cultural identity and value, COVID-19-related media use, and psychological distress of a group of relatively young and highly educated U.S.-dwelling Chinese (N = 1,256) between March and April 2020, we found that identification with both U.S. and Chinese cultures alleviated immigrants and sojourners' psychological distress. Further, COVID-19-related media use served as a stressor during the pandemic, and perceived hostility from China led to stronger psychological distress among U.S.-dwelling Chinese.

The Search Between Two Worlds: Motivations for and Consequences of U.S.-Dwelling Chinese's Use of U.S. and Chinese Media for COVID-19 Information.

As the COVID-19 pandemic continues to inflict damage throughout the world, some minority groups are bearing a disproportionate share of its impacts. We concentrated on one such group, U.S.-dwelling Chinese, who have had to cope with challenges related to acculturation, health, safety, and racism. Recognizing that health information seeking was an essential step in helping maintain and improve health behaviors, we conducted a two-wave longitudinal study (N = 1,284) to examine the various factors predicting U.S.-dwelling Chinese's use of U.S. and Chinese media for COVID-19 information as well as the consequences of their information seeking. Overall, we found that acculturation, accuracy (i.e., information insufficiency) and defense (i.e., conspiratorial beliefs) motivations, trust in media, and perceived information gathering capacity played a key role in explaining information seeking from an intercultural viewpoint, and that the use of U.S. and Chinese media was associated with different health behaviors. These findings contribute to theory and practice in a variety of ways.

Comparative transcriptomic analyzes of human lung epithelial cells infected with wild-type SARS-CoV-2 and its variant with a 12-bp missing in the E gene.

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel coronavirus that caused a global outbreak of coronavirus disease 2019 (COVID-19) pandemic. To elucidate the mechanism of SARS-CoV-2 replication and immunogenicity, we performed a comparative transcriptome profile of mRNA and long non-coding RNAs (lncRNAs) in human lung epithelial cells infected with the SARS-CoV-2 wild-type strain (8X) and the variant with a 12-bp deletion in the E gene (F8). In total, 3,966 differentially expressed genes (DEGs) and 110 differentially expressed lncRNA (DE-lncRNA) candidates were identified. Of these, 94 DEGs and 32 DE-lncRNAs were found between samples infected with F8 and 8X. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyzes revealed that pathways such as the TNF signaling pathway and viral protein interaction with cytokine and cytokine receptor were involved. Furthermore, we constructed a lncRNA-protein-coding gene co-expression interaction network. The KEGG analysis of the co-expressed genes showed that these differentially expressed lncRNAs were enriched in pathways related to the immune response, which might explain the different replication and immunogenicity properties of the 8X and F8 strains. These results provide a useful resource for studying the pathogenesis of SARS-CoV-2 variants.

Comparative transcriptomic analyzes of human lung epithelial cells infected with wild-type SARS-CoV-2 and its variant with a 12-bp missing in the E gene

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel coronavirus that caused a global outbreak of coronavirus disease 2019 (COVID-19) pandemic. To elucidate the mechanism of SARS-CoV-2 replication and immunogenicity, we performed a comparative transcriptome profile of mRNA and long non-coding RNAs (lncRNAs) in human lung epithelial cells infected with the SARS-CoV-2 wild-type strain (8X) and the variant with a 12-bp deletion in the E gene (F8). In total, 3,966 differentially expressed genes (DEGs) and 110 differentially expressed lncRNA (DE-lncRNA) candidates were identified. Of these, 94 DEGs and 32 DE-lncRNAs were found between samples infected with F8 and 8X. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyzes revealed that pathways such as the TNF signaling pathway and viral protein interaction with cytokine and cytokine receptor were involved. Furthermore, we constructed a lncRNA-protein-coding gene co-expression interaction network. The KEGG analysis of the co-expressed genes showed that these differentially expressed lncRNAs were enriched in pathways related to the immune response, which might explain the different replication and immunogenicity properties of the 8X and F8 strains. These results provide a useful resource for studying the pathogenesis of SARS-CoV-2 variants.

No Laughing Matter: Exploring the Effects of Scientists’ Humor Use on Twitter and the Moderating Role of Superiority

This study explores the effects of two humor styles?pure humor and satire?on audiences? Twitter engagement intentions as well as their attitudes toward mRNA Covid-19 vaccines. In an online between-subjects experiment (N = 484), these styles were embedded within a scientist?s tweet about the vaccines. The results showed that, compared to a no humor tweet, both pure humor and satire increased audience?s expectancy violation, subsequently decreasing both outcomes. Superiority as an individual trait also moderated the relationship between humor styles and both expectancy violation and Twitter engagement. These findings broaden our understanding of different humor styles in science communication on Twitter.

Appendicitis as a Leading Manifestation of Kawasaki Disease in Older Children.

Kawasaki disease (KD) is a systematic inflammatory disease with multiple organ involvement. Timely diagnosis and prompt management are essential for successful treatment. KD, with an atypical presentation, remains a diagnostic challenge for physicians. We report a five-year-old boy who presented with appendicitis. An appendectomy was performed; however, his fever persisted. The boy was diagnosed with KD and intravenous immunoglobulin was administered. His symptoms resolved, and he had an uneventful recovery. Furthermore, we performed a literature review with 13 cases identified in the literature. Most cases were male, and the average age was older than typical for KD. In conclusion, KD may present with abdominal complaints and appendicitis may be a rare initial presentation of KD. Multidisciplinary cooperation and high awareness are warranted for timely diagnosis, especially in older children experiencing persistent fever after an appendectomy.

Development of a Recombinant RBD Subunit Vaccine for SARS-CoV-2.

The novel coronavirus pneumonia (COVID-19) pandemic is a great threat to human society and now is still spreading. Although several vaccines have been authorized for emergency use, only one recombinant subunit vaccine has been permitted for widespread use. More subunit vaccines for COVID-19 should be developed in the future. The receptor binding domain (RBD), located at the S protein of SARS-CoV-2, contains most of the neutralizing epitopes. However, the immunogenicity of RBD monomers is not strong enough. In this study, we fused the RBD-monomer with a modified Fc fragment of human IgG1 to form an RBD-Fc fusion protein. The recombinant vaccine candidate based on the RBD-Fc protein could induce high levels of IgG and neutralizing antibody in mice, and these could last for at least three months. The secretion of IFN-γ, IL-2 and IL-10 in the RBD-stimulated splenocytes of immunized mice also increased significantly. Our results first showed that the RBD-Fc vaccine could induce both humoral and cellular immune responses and might be an optional strategy to control COVID-19.

Experience, experts, statistics, or just science? Predictors and consequences of reliance on different evidence types during the COVID-19 infodemic.

As an unprecedented global disease outbreak, the COVID-19 pandemic is also accompanied by an infodemic. To better cope with the pandemic, laypeople need to process information in ways that help guide informed judgments and decisions. Such information processing likely involves the reliance on various evidence types. Extending the Risk Information Seeking and Processing model via a two-wave survey (N = 1284), we examined the predictors and consequences of US-dwelling Chinese's reliance on four evidence types (i.e. scientific, statistical, experiential, and expert) regarding COVID-19 information. Overall, Risk Information Seeking and Processing variables such as information insufficiency and perceived information gathering capacity predicted the use of all four evidence types. However, other Risk Information Seeking and Processing variables (e.g. informational subjective norms) did not emerge as important predictors. In addition, different evidence types had different associations with subsequent disease prevention behaviors and satisfaction with the US government's action to address the pandemic. Finally, discrete emotions varied in their influences on the use of evidence types, behaviors, and satisfaction. The findings provide potentially valuable contributions to science and health communication theory and practice.

A SARS-CoV-2 variant with the 12-bp deletion at E gene.

The coronavirus disease 2019 (COVID-19) pandemic is still ongoing and has become an important public health threat. This disease is caused by a new coronavirus named severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection, and so far, little is known about this virus. In this study, by using plaque purification, we purified two SARS-CoV-2 virus strains from the same specimen, one named F8 containing a 12-bp deletion in the E gene and the other named 8X containing the wild-type E gene. There was no significant difference in the viral titer and infectivity of these two strains. The S protein content of the F8 viral culture was 0.39 µg/ml, much higher than that of 8X. An inactivated vaccine made from the F8 strain could trigger high levels of the IgG titer and neutralizing antibody titer, which could last for at least 6 weeks and were significantly higher than those from the 8X strain at 1 and 3 weeks post vaccination, respectively. In conclusion, we reported that both the E gene mutant and wild-type SARS-CoV-2 strains were isolated from the same clinical sample by plaque purification. A 12-bp deletion in the E gene was important for SARS-CoV-2 replication and immunogenicity.